1C92

Endo-Beta-N-Acetylglucosaminidase H, E132A Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-01-01
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	44.160, 55.530, 46.680
Unit cell angles	90.00, 104.12, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.218 *
Rwork	0.210
RMSD bond length	0.012
RMSD bond angle	25.690 *
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.051 *
Total number of observations	61772 *
Number of reflections	16076
Completeness [%]	93.3 *	90.2
Redundancy	3.84 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	24% PEG1500, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein		0.003ml
2	1	drop	reservoir		0.004ml
3	1	reservoir	PEG1500	24 (%)