1C91

Endo-Beta-N-Acetylglucosaminidase H, E132D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-01-01
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	61.400, 61.400, 140.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.215 *
Rwork	0.213
RMSD bond length	0.011
RMSD bond angle	24.980 *
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.066 *
Total number of observations	51775 *
Number of reflections	17227
Completeness [%]	87.6 *	83 *
Redundancy	1.89 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	15% PEG1000, 100 MM ZN(AC)2 100 MM CACODYLATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein		0.002ml
2	1	drop	chitobiose		50 times consentration of protein
3	1	drop	reservoir		0.002ml
4	1	reservoir	cacodylate	100 (mM)
5	1	reservoir		100 (mM)
6	1	reservoir	PEG1000	15 (%)