1C91
Endo-Beta-N-Acetylglucosaminidase H, E132D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-01-01 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 61.400, 61.400, 140.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.215 * |
Rwork | 0.213 |
RMSD bond length | 0.011 |
RMSD bond angle | 24.980 * |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.066 * | |
Total number of observations | 51775 * | |
Number of reflections | 17227 | |
Completeness [%] | 87.6 * | 83 * |
Redundancy | 1.89 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 15% PEG1000, 100 MM ZN(AC)2 100 MM CACODYLATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.002ml | |
2 | 1 | drop | chitobiose | 50 times consentration of protein | |
3 | 1 | drop | reservoir | 0.002ml | |
4 | 1 | reservoir | cacodylate | 100 (mM) | |
5 | 1 | reservoir | 100 (mM) | ||
6 | 1 | reservoir | PEG1000 | 15 (%) |