1C90

Endo-Beta-N-Acetylglucosaminidase H, E132Q Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-01-01
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1
Unit cell lengths	47.959, 54.355, 47.632
Unit cell angles	89.68, 109.16, 83.93

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.212 *
Rwork	0.200
RMSD bond length	0.013
RMSD bond angle	25.180 *
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.076 *
Number of reflections	27256
Completeness [%]	91.0 *	88 *
Redundancy	3.00 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	19% PEG1000, 100 MM ZN(AC)2 100 MM CACODYLATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein		0.004ml
2	1	drop	reservoir		0.003ml
3	1	drop	OBP	20 (mM)
4	1	drop	water		0.004ml
5	1	reservoir	cacodylate	100 (mM)
6	1	reservoir		100 (mM)
7	1	reservoir	PEG1000	19 (%)