1C90
Endo-Beta-N-Acetylglucosaminidase H, E132Q Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-01-01 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 |
Unit cell lengths | 47.959, 54.355, 47.632 |
Unit cell angles | 89.68, 109.16, 83.93 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.212 * |
Rwork | 0.200 |
RMSD bond length | 0.013 |
RMSD bond angle | 25.180 * |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.076 * | |
Number of reflections | 27256 | |
Completeness [%] | 91.0 * | 88 * |
Redundancy | 3.00 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 19% PEG1000, 100 MM ZN(AC)2 100 MM CACODYLATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.004ml | |
2 | 1 | drop | reservoir | 0.003ml | |
3 | 1 | drop | OBP | 20 (mM) | |
4 | 1 | drop | water | 0.004ml | |
5 | 1 | reservoir | cacodylate | 100 (mM) | |
6 | 1 | reservoir | 100 (mM) | ||
7 | 1 | reservoir | PEG1000 | 19 (%) |