1C8Y

Endo-Beta-N-Acetylglucosaminidase H, D130A Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-01-01
Detector	RIGAKU RAXIS II
Spacegroup name	P 43 21 2
Unit cell lengths	61.400, 61.400, 140.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.209 *
Rwork	0.207
RMSD bond length	0.010
RMSD bond angle	24.880 *
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.061 *
Total number of observations	41776 *
Number of reflections	13418
Completeness [%]	98.8 *	96.2
Redundancy	3.11 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% PEG1000, 100 MM ZN(AC)2, 100 MM CACODYLATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein		0.002ml
2	1	drop	chitobiose		50 times consentration of protein
3	1	drop	reservoir		0.002ml
4	1	reservoir	cacodylate	100 (mM)
5	1	reservoir		100 (mM)
6	1	reservoir	PEG1000	25 (%)