1C8Q

STRUCTURE SOLUTION AND REFINEMENT OF THE RECOMBINANT HUMAN SALIVARY AMYLASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	195
Detector technology	IMAGE PLATE
Collection date	1995-07-01
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	52.707, 74.768, 133.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.990 - 2.300
Rwork	0.173
R-free	0.21600
Structure solution method	MOLECULARE REPLACEMENT
Starting model (for MR)	1smd
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	9.990
High resolution limit [Å]	2.200	2.300
Rmerge	0.083
Number of reflections	30708
Completeness [%]		90

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	273	10 mM Tris- HCL containing 5 mM CaCl2, 44% MPD, protein concentration 20 mg/ml, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 273K