1C8E

FELINE PANLEUKOPENIA VIRUS EMPTY CAPSID STRUCTURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-01-15
Detector	ADSC QUANTUM 4
Spacegroup name	P 1 21 1
Unit cell lengths	254.154, 253.962, 380.288
Unit cell angles	90.00, 92.83, 90.00

Refinement procedure

Resolution	9.000 - 3.000
R-factor	0.288
RMSD bond length	0.010
RMSD bond angle	1.680
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	GLRF
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.092	0.221
Number of reflections	810220
<I/σ(I)>	12.3
Completeness [%]	89.2	47.3
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	19 *	0.75-1.5% PEG8000, 5 mM EDTA, 20 mM BIS-Tris pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	0.75-1.50 (%(w/v))
2	1	reservoir		8 (mM)	or 5mM EDTA
3	1	drop	virus	10 (mg/ml)
4	1	drop	Bis-Tris	20 (mM)
5	1	drop	Tris-HCl	20 (mM)