1C8C

CRYSTAL STRUCTURES OF THE CHROMOSOMAL PROTEINS SSO7D/SAC7D BOUND TO DNA CONTAINING T-G MISMATCHED BASE PAIRS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	123
Detector technology	IMAGE PLATE
Collection date	1998-08-15
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	47.475, 49.800, 37.681
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.450
R-factor	0.229
R-free	0.28680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1azp
RMSD bond length	0.009 *
RMSD bond angle	2.300 *
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.380	1.450 *
Rmerge	0.054
Number of reflections	14498
Completeness [%]	89.1	63 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5		PROTEIN WAS CRYSTALLIZED FROM 1.3MM SSO7D, 1.3MM DUPLEX DNA, 2.5 MM TRIS (PH 6.5), 2.5% PEG 400, EQUILIBRATED AGAINST 15% PEG 400 at pH 6.5, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	TRIS
2	1	1	PEG 400
3	1	2	PEG 400

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Sso7d	1.3 (mM)
2	1	drop	DNA duplex	1.3 (mM)
3	1	drop	Tris-HCl	2.5 (mM)
4	1	drop	PEG400	2.5 (%)
5	1	reservoir	PEG400	15 (%)