1C80

REGULATORY COMPLEX OF FRUCTOSE-2,6-BISPHOSPHATASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	FUJI
Spacegroup name	C 1 2 1
Unit cell lengths	120.800, 51.600, 79.900
Unit cell angles	90.00, 126.10, 90.00

Refinement procedure

Resolution	18.000 - 2.200
Rwork	0.201
R-free	0.26500
RMSD bond length	0.020
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	18.000
High resolution limit [Å]	2.200
Rmerge	0.054
Number of reflections	19365
<I/σ(I)>	6.7
Completeness [%]	94.7
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.25	293	PEG 4000, sodium chloride, Hepes, pH 7.25, EVAPORATION, temperature 293.0K