1C7Y
E.COLI RUVA-HOLLIDAY JUNCTION COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-15 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 158.650, 158.650, 158.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.800 - 3.100 |
| R-factor | 0.25 |
| Rwork | 0.249 |
| R-free | 0.27900 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 20.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.054 | 0.275 |
| Total number of observations | 143269 * | |
| Number of reflections | 7136 | |
| <I/σ(I)> | 47 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20 * | ammonium sulfate, sodium chloride, glycerol, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | glycerol | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | (NH4)2SO4 | ||
| 5 | 1 | 2 | glycerol | ||
| 6 | 1 | 2 | NaCl | ||
| 7 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Mes/NaOH | 0.1 (M) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.0-2.2 (M) | |
| 3 | 1 | reservoir | glycerol | 5 (%) |
