1C7R
THE CRYSTAL STRUCTURE OF PHOSPHOGLUCOSE ISOMERASE/AUTOCRINE MOTILITY FACTOR/NEUROLEUKIN COMPLEXED WITH ITS CARBOHYDRATE PHOSPHATE INHIBITORS AND ITS SUBSTRATE RECOGNITION MECHANISM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-01 |
| Detector | RIGAKU |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 74.800, 94.710, 171.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.970 - 2.500 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pgi |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.970 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.099 | 0.615 * |
| Total number of observations | 87547 * | |
| Number of reflections | 21464 | |
| <I/σ(I)> | 13.4 | 2.2 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 4.08 | 4.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 0.1M HEPES PH 7.5, 0.8M K, NA TARTRATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 5PA | 0.05 (mM) | |
| 3 | 1 | drop | phosphate | 50 (mM) | |
| 4 | 1 | reservoir | ammonium dihydrogen phosphate | 0.4 (M) |
