1C7P
CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME WITH FOUR EXTRA RESIDUES (EAEA) AT THE N-TERMINAL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 283 |
Detector technology | DIFFRACTOMETER |
Collection date | 1998-12-15 |
Detector | WEISSENBERG |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 91.080, 91.080, 92.730 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 5.000 - 2.400 |
R-factor | 0.197 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.648 |
Data reduction software | DENZO |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.400 |
Rmerge | 0.044 |
Total number of observations | 72761 * |
Number of reflections | 9367 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 10 * | equal volume of protein and reservoir solution were used for the drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | cadmium chloride | 0.1 (M) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
4 | 1 | reservoir | PEG400 | 30 (%(v/v)) |