1C7N

CRYSTAL STRUCTURE OF CYSTALYSIN FROM TREPONEMA DENTICOLA CONTAINS A PYRIDOXAL 5'-PHOSPHATE COFACTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	1998-11-08
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	89.470, 108.440, 176.090
Unit cell angles	90.00, 90.17, 90.00

Refinement procedure

Resolution	25.000 - 1.900
R-factor	0.215
Rwork	0.208
R-free	0.24700
RMSD bond length	0.006 *
RMSD bond angle	1.400 *

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.078	0.255
Total number of observations	382434 *
Number of reflections	254989
<I/σ(I)>	8.6
Completeness [%]	96.5	87.9
Redundancy	1.5	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	20 *	PEG 4000, magnesium acetate, MES, pH 6.5, vapor diffusion/sitting drop, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	15 (%(w/v))
3	1	reservoir	magnesium acetate	0.2 (M)
4	1	reservoir	MES-NaOH	100 (mM)