1C7K

CRYSTAL STRUCTURE OF THE ZINC PROTEASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1997-12-18
Detector	RIGAKU RAXIS
Spacegroup name	P 21 21 21
Unit cell lengths	55.103, 55.199, 37.243
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.000 - 1.000
R-factor	0.148
R-free	0.17000 *
Structure solution method	AB INITIO
RMSD bond length	0.014 *
RMSD bond angle	0.029 *
Phasing software	SHELXS
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	0.970
High resolution limit [Å]	0.920	0.920
Rmerge	0.057
Total number of observations	158614 *
Number of reflections	51419 *
<I/σ(I)>	14.64
Completeness [%]	82.0 *	58.3
Redundancy	58.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7	4 *	Harada, S., (1991) J.BIOCHEM.(TOKYO), 110, 46. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	calcium acetate	0.1 (M)
2	1	1	protein	5-10 (mg/ml)
3	1	1	acetone	40 (%(v/v))