Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-04-12 |
| Detector | ADSC QUANTUM |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.507, 81.833, 98.837 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.600 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.22800 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.048 | 0.318 |
| Number of reflections | 67818 | |
| <I/σ(I)> | 40.4 | |
| Completeness [%] | 93.0 | 61.4 |
| Redundancy | 8.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.4 | 18 * | 29% PEG 4000, 100MM TRIS, 175 LISO4, 10MM DMF, pH 8.40 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 29 (%) | |
| 3 | 1 | reservoir | Tris | 100 (mM) | |
| 4 | 1 | reservoir | 160-185 (mM) | ||
| 5 | 1 | reservoir | dimethylformamide | 8-12 (%) |
