1C7E
D95E HYDROQUINONE FLAVODOXIN MUTANT FROM D. VULGARIS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1998-11-01 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.041, 61.508, 75.559 |
Unit cell angles | 90.00, 127.06, 90.00 |
Refinement procedure
Resolution | 12.000 * - 2.250 |
R-factor | 0.2308 * |
Rwork | 0.227 |
R-free | 0.29400 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 0.047 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.764 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.660 | 0.314 |
Total number of observations | 35676 * | |
Number of reflections | 15535 | |
<I/σ(I)> | 8.8 | 2.4 |
Completeness [%] | 95.4 | 92.9 |
Redundancy | 2.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 * | 18 * | 70-73% A.S., 210MM TRIS-HCL, 200MM SODIUM ACETATE PH=8.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12-15 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 30-35 (%sat) | |
3 | 1 | drop | Tris-HCl | 50 (mM) | or 25mM sodium phosphate containing 1mM EDTA |
4 | 1 | reservoir | ammonium sulfate | 60-70 (%sat) |