1C76
STAPHYLOKINASE (SAK) MONOMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-28 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.920, 59.840, 102.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.216 |
| Rwork | 0.201 |
| R-free | 0.27300 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.010 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.350 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.043 | 0.182 |
| Number of reflections | 6589 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 97.8 | 97.6 |
| Redundancy | 4.4 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 8000, pH 8.0, vapor diffusion/hanging drop, temperature 293K |
