1C6V
SIV INTEGRASE (CATALYTIC DOMAIN + DNA BIDING DOMAIN COMPRISING RESIDUES 50-293) MUTANT WITH PHE 185 REPLACED BY HIS (F185H)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-02-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.570, 100.000, 150.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 3.000 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.36200 |
Structure solution method | SIR + MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ITG AND 1IHV |
RMSD bond length | 0.009 |
RMSD bond angle | 2.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.113 | 0.608 |
Total number of observations | 309679 * | |
Number of reflections | 22129 | |
<I/σ(I)> | 1.5 | |
Completeness [%] | 92.5 | 66.8 |
Redundancy | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 5.7 | 0.1 M MES, PH=5.7, PEG6K 8%, 15% DIOXANE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | Mes | 0.1 (M) | |
2 | 1 | 1 | PEG6000 | 8 (%(v/v)) | |
3 | 1 | 1 | dioxane | 1.5 (%(v/v)) |