1C6R

CRYSTAL STRUCTURE OF REDUCED CYTOCHROME C6 FROM THE GREEN ALGAE SCENEDESMUS OBLIQUUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	291
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	35.560, 48.470, 43.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.1995 *
Rwork	0.200
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cyj
RMSD bond length	0.012
RMSD bond angle	22.300 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.140 *	0.383 *
Total number of observations	34521 *
Number of reflections	8505
<I/σ(I)>	3.5	1.8
Completeness [%]	99.4	95.1
Redundancy	4.0 *	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		150 (mM)
2	1	reservoir	PEG4000	20 (%(w/v))
3	1	reservoir	Tris-HCl	5 (mM)