1C68

T4 LYSOZYME MUTANT C54T/C97A/L121A/L133A IN THE PRESENCE OF 8 ATM XENON

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	273
Detector technology	IMAGE PLATE
Collection date	1998-02-10
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 32 2 1
Unit cell lengths	61.126, 61.126, 96.716
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.000 - 2.500
Starting model (for MR)	251l
RMSD bond length	0.020
RMSD bond angle	16.480 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.062	0.266
Number of reflections	6933
<I/σ(I)>	9.2
Completeness [%]	86.0 *	88.4
Redundancy	2.02

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *			1.8-2.2 M NAH2/K2HPO4, PH 6.9-7.1, 50 MM BETA-MERCAPTOETHANOL AND/OR 50 MM HYDROXYETHYL DISULFIDE