1C64
T4 LYSOZYME MUTANT C54T/C97A/L121A IN THE PRESENCE OF 8 ATM KRYPTON
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 273 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05-14 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.189, 61.189, 96.914 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.000 - 2.000 |
| Starting model (for MR) | 200l |
| RMSD bond length | 0.020 |
| RMSD bond angle | 15.360 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.046 | 0.219 |
| Number of reflections | 14141 | |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 96.0 * | 98.9 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.7 * | Remington, S.J., (1978) J.Mol.Biol., 118, 81. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 2 | 1 | 1 | 0.55 (M) | ||
| 3 | 1 | 1 | mercaptoethanol | 14 (mM) | |
| 4 | 1 | 1 | 1 (mM) | ||
| 5 | 1 | 1 | sodium phosphate | 0.01 (M) | |
| 6 | 1 | 1 | 2.2 (M) | ||
| 7 | 1 | 1 | 1.8 (M) |
