1C5K
THE STRUCTURE OF TOLB, AN ESSENTIAL COMPONENT OF THE TOL-DEPENDENT TRANSLOCATION SYSTEM AND ITS INTERACTIONS WITH THE TRANSLOCATION DOMAIN OF COLICIN E9
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 1999-04-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.91, 1.07, 1.386, 1.387 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.580, 40.190, 77.660 |
Unit cell angles | 90.00, 110.22, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.27100 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 17.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.052 * | 0.176 * |
Number of reflections | 59093 | |
Completeness [%] | 98.4 | 97.4 |
Redundancy | 2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.5 * | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 5 (mg/ml) | |
2 | 1 | 1 | Tris-HCl | 10 (mM) | |
3 | 1 | 2 | PEG8000 | 18 (%(w/v)) | |
4 | 1 | 2 | MES | 100 (mM) |