1C4T

CATALYTIC DOMAIN FROM TRIMERIC DIHYDROLIPOAMIDE SUCCINYLTRANSFERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-08
Detector	RIGAKU RAXIS IV
Spacegroup name	P 31 2 1
Unit cell lengths	112.175, 112.175, 134.417
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 3.000
R-factor	0.257
Rwork	0.257
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e2o
RMSD bond length	0.007
RMSD bond angle	25.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.103 *	0.309 *
Number of reflections	18677 *	1798 *
<I/σ(I)>	13.8	2.6
Completeness [%]	95.8 *	93.5
Redundancy	2.9	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	HEPES	100 (mM)
3	1	reservoir	sodium acetate	1 (M)
4	1	reservoir	cadmium sulfate	50 (mM)