1C4O
CRYSTAL STRUCTURE OF THE DNA NUCLEOTIDE EXCISION REPAIR ENZYME UVRB FROM THERMUS THERMOPHILUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 138 |
| Detector technology | CCD |
| Collection date | 1999-06-01 |
| Detector | SBC |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 134.740, 134.740, 106.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.900 - 1.500 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.26600 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 23.000 * |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS (0.5) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.820 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.070 | 0.729 |
| Number of reflections | 176690 | |
| <I/σ(I)> | 26 | 1.3 |
| Completeness [%] | 99.6 | 93.3 |
| Redundancy | 7.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | Shibata, A., (1999) Acta Crystallogr.D55, 704. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | beta-mercaptoehtanol | 10 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 5 | 1 | drop | Tris-HCl | 50 (mM) | |
| 6 | 1 | reservoir | lithium sulfate | 1.78 (M) | |
| 7 | 1 | reservoir | beta-octylglucoside | 0.5 (%(w/v)) | |
| 8 | 1 | reservoir | MES | 100 (mM) |
