1C46
MUTANT HUMAN LYSOZYME WITH FOREIGN N-TERMINAL RESIDUES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-15 |
| Detector | RIGAKU |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 56.558, 56.558, 116.485 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WILD-TYPE HUMAN LYSOZYME |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.570 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.096 |
| Total number of observations | 19835 * |
| Number of reflections | 9683 |
| Completeness [%] | 71.9 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | pH 4.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 1.6-1.9 (M) | ||
| 2 | 1 | reservoir | acetate | 20 (mM) |
