Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-04-15 |
| Detector | SIEMENS X200B |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 81.497, 81.497, 107.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.900 - 2.300 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mhb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 19.300 * |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | PHASER |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.900 | |
| High resolution limit [Å] | 2.300 | 2.300 * |
| Rmerge | 0.072 | |
| Total number of observations | 28271 * | |
| Number of reflections | 15772 | |
| Completeness [%] | 95.0 | 70.1 * |
| Redundancy | 2.66 * | 1.84 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 | 291 * | pH 6.80 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | potassium phosphate | 2.5 (mM) | |
| 2 | 1 | drop | protein | 25-30 (mg/ml) | |
| 3 | 1 | drop | PEG6000 | 4 (%) | |
| 4 | 1 | drop | potassium phosphate | 15 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 12 (%) |
