1C3X
PURINE NUCLEOSIDE PHOSPHORYLASE FROM CELLULOMONAS SP. IN COMPLEX WITH 8-IODO-GUANINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-01-28 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.319, 108.340, 117.562 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.000 - 2.400 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.25000 * |
RMSD bond length | 0.015 * |
RMSD bond angle | 2.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.000 | 2.420 |
High resolution limit [Å] | 2.250 * | 2.250 |
Rmerge | 0.081 | 0.444 |
Number of reflections | 35723 | |
<I/σ(I)> | 9.3 | |
Completeness [%] | 90.0 | 64 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7 * | 298 | PEG 4000, CACODYLATE, CA-ACETATE, SODIUM PHOSPHATE, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | PEG4000 | 8-15 (%(w/v)) | |
2 | 1 | 1 | cacodylate | 50 (mM) | |
3 | 1 | 1 | calcium acetate | 100 (mM) | |
4 | 1 | 2 | protein | 7-11 (mg/ml) | |
5 | 1 | 2 | sodium phosphate | 6-25 (mM) |