1C3U
T. MARITIMA ADENYLOSUCCINATE LYASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-21 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 120.498, 126.213, 169.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| Rwork | 0.199 |
| R-free | 0.23000 |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 2.840 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.040 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.085 | 0.263 |
| Number of reflections | 57257 | |
| <I/σ(I)> | 22.67 | |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 5.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 * | 18 * | 100 mM Bicine, 5 mM AMP, 1.8 M Ammonium Sulfate, pH 9.00, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12.5 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 3 | 1 | reservoir | PEG4000 | 0.1-0.5 (%) | |
| 4 | 1 | reservoir | glycerol | 6 (%(v/v)) | |
| 5 | 1 | reservoir | beta-mercaptoethanol | 1 (mM) | |
| 6 | 1 | reservoir | 0.02 (%(w/v)) |
