1C3J
T4 PHAGE BETA-GLUCOSYLTRANSFERASE: SUBSTRATE BINDING AND PROPOSED CATALYTIC MECHANISM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 151.700, 52.260, 52.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.880 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.23300 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.788 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | FFT |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 1.980 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.050 | 0.278 |
| Total number of observations | 223460 * | |
| Number of reflections | 31888 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 92.0 | 71 |
| Redundancy | 6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | AMMONIUM SULFATE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 26 (%sat) | |
| 3 | 1 | drop | UDP | 1 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 52 (%sat) | |
| 5 | 1 | reservoir | MES | 0.1 (M) |
