1C3F

Endo-Beta-N-Acetylglucosaminidase H, D130N Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-01-01
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	84.000, 84.000, 88.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.218 *
Rwork	0.209
RMSD bond length	0.011
RMSD bond angle	25.140 *
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.052 *
Number of reflections	17755
Completeness [%]	95.3 *	74.4
Redundancy	6.60 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	17% PEG8000, 200 MM ZINC ACETATE 100 MM CACODYLATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein		0.004ml
2	1	drop	reservoir		0.003ml
3	1	drop	OBG	20 (mM)
4	1	drop	water		0.004ml
5	1	reservoir	cacodylate	100 (mM)
6	1	reservoir		200 (mM)
7	1	reservoir	PEG8000	17 (%)