1C2R

MOLECULAR STRUCTURE OF CYTOCHROME C2 ISOLATED FROM RHODOBACTER CAPSULATUS DETERMINED AT 2.5 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	H 3 2
Unit cell lengths	100.030, 100.030, 162.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	100.000 * - 2.500
R-factor	0.168
RMSD bond length	0.016
RMSD bond angle	2.635
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.500 *
Rmerge	0.039 *
Total number of observations	12788 *
Number of reflections	9496 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	3.2-3.5 (M)
2	1	reservoir		250 (mM)
3	1	reservoir	sodium potassium phosphate	50 (mM)
4	1	drop	protein	10-30 (mg/ml)
5	1	drop	sodium potassium phospahte	10 (mM)