1C25
HUMAN CDC25A CATALYTIC DOMAIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 41 |
| Unit cell lengths | 43.507, 43.507, 117.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.000 - 2.300 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.29600 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 24.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.180 | |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.067 | 0.372 |
| Total number of observations | 39374 * | |
| Number of reflections | 12551 | |
| <I/σ(I)> | 18 | 3 |
| Completeness [%] | 98.3 | 100 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 4 * | 4 MICROLITERS PROTEIN (10 MG/ML IN 200 MM NACL, 1 MM DTT, 20 MM HEPES, PH 7.4) MIXED WITH 4 MICROLITERS WELL BUFFER (18-20% PEG 3350, 0.025% BETA-OCTYLGLUCOSIDE, 0.11 M SODIUM CITRATE, PH 5.8) |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | HEPES | 20 (mM) | |
| 5 | 1 | reservoir | PEG3350 | 18-20 (%) | |
| 6 | 1 | reservoir | beta-octylglucoside | 0.025 (%) | |
| 7 | 1 | reservoir | sodium citrate | 0.11 (M) |
