1C25

HUMAN CDC25A CATALYTIC DOMAIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1997-08
Detector	RIGAKU RAXIS IV
Spacegroup name	P 41
Unit cell lengths	43.507, 43.507, 117.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.000 - 2.300
R-factor	0.227
Rwork	0.227
R-free	0.29600
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	24.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.067	0.372
Total number of observations	39374 *
Number of reflections	12551
<I/σ(I)>	18	3
Completeness [%]	98.3	100
Redundancy	3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *	4 *	4 MICROLITERS PROTEIN (10 MG/ML IN 200 MM NACL, 1 MM DTT, 20 MM HEPES, PH 7.4) MIXED WITH 4 MICROLITERS WELL BUFFER (18-20% PEG 3350, 0.025% BETA-OCTYLGLUCOSIDE, 0.11 M SODIUM CITRATE, PH 5.8)

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		200 (mM)
3	1	drop	dithiothreitol	1 (mM)
4	1	drop	HEPES	20 (mM)
5	1	reservoir	PEG3350	18-20 (%)
6	1	reservoir	beta-octylglucoside	0.025 (%)
7	1	reservoir	sodium citrate	0.11 (M)