1C23
E. COLI METHIONINE AMINOPEPTIDASE: METHIONINE PHOSPHONATE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-27 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.221, 67.518, 48.823 |
| Unit cell angles | 90.00, 111.20, 90.00 |
Refinement procedure
| Resolution | 32.200 - 2.000 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.160 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.200 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.064 | 0.200 |
| Total number of observations | 79515 * | |
| Number of reflections | 16137 * | |
| <I/σ(I)> | 27.4 | 6.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 * | 298 | Hepes, NaCl, K2SO4, CoCl2, PEG 4000, methionine, N-octanoyl sucrose, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 10 | 1 | reservoir | 2 (mM) | ||
| 2 | 1 | drop | HEPES | 25 (mM) | |
| 3 | 1 | drop | 25 (mM) | ||
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | drop | 1 (mM) | ||
| 6 | 1 | drop | methionine | 15 (mM) | |
| 7 | 1 | drop | N-octanoyl sucrose | 48.8 (mM) | |
| 8 | 1 | reservoir | PEG4000 | 24-26 (%) | |
| 9 | 1 | reservoir | HEPES | 0.1 (M) |
