1C1G
CRYSTAL STRUCTURE OF TROPOMYOSIN AT 7 ANGSTROMS RESOLUTION IN THE SPERMINE-INDUCED CRYSTAL FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 21 |
| Detector technology | CCD |
| Wavelength(s) | 1.000, 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 259.738, 55.300, 136.259 |
| Unit cell angles | 90.00, 97.42, 90.00 |
Refinement procedure
| Resolution | 100.000 - 7.000 |
| Rwork | 0.404 |
| Structure solution method | SIR |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 7.000 |
| Number of reflections | 3117 |
| Completeness [%] | 96.4 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7.4 | 15-17.5 * | SPERMINE, pH 7.4, LIQUID DIFFUSION, temperature 18K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20-30 (mg/ml) | |
| 2 | 1 | 1 | spermine | 0.040-0.065 (M) | |
| 3 | 1 | 2 | spermine | 0.070 (M) |
