1C1A
CRYSTAL STRUCTURE OF RSV TWO-DOMAIN INTEGRASE
Experimental procedure
| Experimental method | MULTIPLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 1998-11-17 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.240, 46.339, 94.309 |
| Unit cell angles | 90.00, 101.76, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.100 |
| R-factor | 0.256 |
| Rwork | 0.256 |
| R-free | 0.33700 |
| Structure solution method | MAD PHASING |
| Starting model (for MR) | ASV INTEGRASE CATALYTIC DOMAIN (1VSE) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.060 | 0.177 |
| Number of reflections | 11250 | |
| <I/σ(I)> | 15.6 | 5.5 |
| Completeness [%] | 98.6 | 96.5 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 0-5 * | ROD-SHAPED CRYSTALS WERE GROWN BY SITTING DROP VAPOR DIFFUSION METHOD. RESERVOIR SOLUTION CONTAINED 0.050M KCL, 0.01M MGCL2, 0.1M SODIUM CACODYLATE, 0.02M IMIDAZOLE, PH 7.2, 10% PEG3350, AND 20% (V/V) ETHYLENE GLYCOL. THE DROP CONTAINED 1:1 MIXTURE OF RESERVOIR SOLUTION AND PROTEIN (10-15 MG/ML) |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.05 (M) | ||
| 2 | 1 | reservoir | 0.01 (M) | ||
| 3 | 1 | reservoir | sodium cacodylate | 0.05 (M) | |
| 4 | 1 | reservoir | PEG3350 | 10 (%(w/v)) | |
| 5 | 1 | reservoir | ethylene glycol | 25 (%(v/v)) |
