1C16
CRYSTAL STRUCTURE ANALYSIS OF THE GAMMA/DELTA T CELL LIGAND T22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 167.110, 91.470, 122.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.100 |
R-factor | 0.245 * |
Rwork | 0.284 |
R-free | 0.30100 * |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.083 | 0.346 |
Number of reflections | 34819 | 2597 * |
<I/σ(I)> | 8.6 | |
Completeness [%] | 90.7 | 69.2 |
Redundancy | 2.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 13 (mg/ml) | |
2 | 1 | 1 | imidazole-maleate | 0.1 (M) | |
3 | 1 | 1 | calcium acetate | 0.2 (M) | |
4 | 1 | 1 | PEG8000 | 10 (%) |