1C0T
CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH BM+21.1326
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 135.100, 112.700, 75.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.208 |
| Rwork | 0.214 |
| R-free | 0.27600 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.067 | 0.285 |
| Total number of observations | 64045 * | |
| Number of reflections | 26072 | |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 81.2 | 64.7 |
| Redundancy | 2.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 4 * | Stammers, D.K., (1994) J.Mol.Biol., 242, 586. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 26 (mg/ml) | |
| 2 | 1 | drop | PEG3400 | 6 (%(w/v)) | |
| 3 | 1 | drop | citrate/phosphate | ||
| 4 | 1 | reservoir | PEG | 6 (%(w/v)) |
