1C0R
COMPLEX OF VANCOMYCIN WITH D-LACTIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-09-20 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 28.440, 28.440, 66.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.000 |
| R-factor | 0.114 |
| R-free | 0.14200 |
| RMSD bond length | 0.019 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.052 | 0.065 |
| Total number of observations | 235187 * | |
| Number of reflections | 13896 | |
| <I/σ(I)> | 29.8 | |
| Completeness [%] | 97.0 | 54 |
| Redundancy | 16.4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | SODIUM CHLORIDE, LACTIC ACID, PH 4.6, VAPOR DIFFUSION/HANGING DROP, TEMPERATURE 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | ligand | 100 (mM) | |
| 3 | 1 | reservoir | 2.0-2.4 (M) |
