1C0F

CRYSTAL STRUCTURE OF DICTYOSTELIUM CAATP-ACTIN IN COMPLEX WITH GELSOLIN SEGMENT 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-04-05
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	57.000, 69.550, 184.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400
Rwork	0.183
R-free	0.23100
RMSD bond length	0.010 *
RMSD bond angle	1.900
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	2.520
High resolution limit [Å]	2.400	2.400
Rmerge	0.074	0.155
Total number of observations	68469 *
Number of reflections	27962
<I/σ(I)>	8
Completeness [%]	94.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	290	IMIDAZOLE, SODIUM CHLORIDE, ATP, CALCIUM CHLORIDE, MAGNESIUM CHLORIDE, SODIUM AZIDE, DITHIOTHREITOL, PEG 6000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
10	1	reservoir		150 (mM)
11	1	reservoir	ATP	0.1 (mM)
12	1	reservoir		0.1 (mM)
13	1	reservoir		0.1 (mM)
14	1	reservoir		1 (mM)
15	1	reservoir	dithiothreitol	10 (mM)
2	1	drop	imidazole-HCl	10 (mM)
3	1	drop		150 (mM)
4	1	drop	ATP	0.1 (mM)
5	1	drop		0.1 (mM)
6	1	drop		0.1 (mM)
7	1	drop		1 (mM)
8	1	drop	dithiothreitol	0.1 (mM)
9	1	reservoir	imidazole-HCl	50 (mM)