1C0C

BOVINE PANCREATIC RIBONUCLEASE A DESICCATED FOR 4.0 DAYS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-09-14
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	35.700, 39.700, 50.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.19
Rwork	0.185
R-free	0.26800
RMSD bond length	0.010
RMSD bond angle	1.270
Data reduction software	bioteX
Data scaling software	bioteX
Phasing software	MERLOT
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *	2.200
High resolution limit [Å]	2.000	2.000
Rmerge	0.045	0.190
Total number of observations	14417 *
Number of reflections	4742
<I/σ(I)>	17.3
Completeness [%]	84.5	64
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	4.5	295	58% methanol (v/v), pH 4.5, SMALL TUBES, temperature 295K