1C0A
CRYSTAL STRUCTURE OF THE E. COLI ASPARTYL-TRNA SYNTHETASE : TRNAASP : ASPARTYL-ADENYLATE COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE D41A |
Synchrotron site | LURE |
Beamline | D41A |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 101.200, 101.200, 231.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.400 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.24900 |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.324 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.000 | |
High resolution limit [Å] | 2.400 | |
Rmerge | 0.059 | 0.214 * |
Number of reflections | 46618 | |
Completeness [%] | 98.1 | 93.2 * |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | AMMONIUM SULFATE, GLYCEROL, BIS-TRIS-PROPANE, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)2SO4 | ||
2 | 1 | 1 | GLYCEROL | ||
3 | 1 | 1 | BIS-TRIS-PROPANE | ||
4 | 1 | 2 | (NH4)2SO4 | ||
5 | 1 | 2 | GLYCEROL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | tRNAAsp | 4.7 (mg/ml) | |
4 | 1 | drop | 2 (mM) | ||
5 | 1 | drop | EDTA | 0.25 (mM) | |
6 | 1 | drop | sodium cacodylate | 5 (mM) | |
7 | 1 | reservoir | glycerol | 15 (%) | |
8 | 1 | reservoir | ammonium sulfate | 2 (M) | |
9 | 1 | reservoir | Bis-Tris-propane-HCl | 50 (mM) |