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1C04

IDENTIFICATION OF KNOWN PROTEIN AND RNA STRUCTURES IN A 5 A MAP OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X12B
Synchrotron siteNSLS
BeamlineX12B
Temperature [K]100
Detector technologyCCD
Collection date1998-03-15
DetectorADSC QUANTUM 4
Spacegroup nameC 2 2 21
Unit cell lengths212.000, 301.400, 576.300
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution60.000 - 5.000
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]130.0003.810
High resolution limit [Å]3.7503.750
Rmerge0.1030.513
Total number of observations957850

*

Number of reflections185190

*

<I/σ(I)>13
Completeness [%]98.696
Redundancy5.24
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

5.6

*

19

*

von Bohlen, K., (1991) J. Mol. Biol., 222, 11.

*

1Vapor diffusion

*

5.6

*

19

*

von Bohlen, K., (1991) J. Mol. Biol., 222, 11.

*

Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111KCL
211NH4CL
311MGCL2
411SODIUM ACETATE
511PEG 6000
612PEG 6000
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein5 (mg/ml)
21drop1.2 (M)
31drop0.005 (M)
41dropPEG60005-6 (%)
51dropacetate0.1 (M)
61drop0.00185 (mM)
71dropbeta-octylglucoside0.0074 (mM)
81reservoir1.7 (M)

227561

PDB entries from 2024-11-20

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