1C04
IDENTIFICATION OF KNOWN PROTEIN AND RNA STRUCTURES IN A 5 A MAP OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-03-15 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 212.000, 301.400, 576.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.000 - 5.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 130.000 | 3.810 |
High resolution limit [Å] | 3.750 | 3.750 |
Rmerge | 0.103 | 0.513 |
Total number of observations | 957850 * | |
Number of reflections | 185190 * | |
<I/σ(I)> | 13 | |
Completeness [%] | 98.6 | 96 |
Redundancy | 5.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5.6 * | 19 * | von Bohlen, K., (1991) J. Mol. Biol., 222, 11. * |
1 | Vapor diffusion * | 5.6 * | 19 * | von Bohlen, K., (1991) J. Mol. Biol., 222, 11. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | KCL | ||
2 | 1 | 1 | NH4CL | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | SODIUM ACETATE | ||
5 | 1 | 1 | PEG 6000 | ||
6 | 1 | 2 | PEG 6000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | 1.2 (M) | ||
3 | 1 | drop | 0.005 (M) | ||
4 | 1 | drop | PEG6000 | 5-6 (%) | |
5 | 1 | drop | acetate | 0.1 (M) | |
6 | 1 | drop | 0.00185 (mM) | ||
7 | 1 | drop | beta-octylglucoside | 0.0074 (mM) | |
8 | 1 | reservoir | 1.7 (M) |