1BZO

THREE-DIMENSIONAL STRUCTURE OF PROKARYOTIC CU,ZN SUPEROXIDE DISMUTASE FROM P.LEIOGNATHI, SOLVED BY X-RAY CRYSTALLOGRAPHY.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-06-02
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	86.890, 86.890, 99.010
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.700 - 2.100
R-free	0.26000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PREVIOUS MODEL OF THE PROTEIN IN SPACE GROUP C2 NOT DEPOSITED IN PDB
RMSD bond length	0.018
RMSD bond angle	2.620
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	TNT (5E)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.700
High resolution limit [Å]	2.100
Rmerge	0.060
Number of reflections	8469
Completeness [%]	96.0
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4	28 *	PEG 8,000 25%, NACL 100 MM, SODIUM ACETATE 50 MM, PH 4, T=28C

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	20 (%(w/v))
3	1	reservoir	sodium chloride	100 (mM)
4	1	reservoir	sodium acetate	50 (mM)