1BZA
BETA-LACTAMASE TOHO-1 FROM ESCHERICHIA COLI TUH12191
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | FUJI |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 73.309, 73.309, 99.374 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 1.800 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2blm |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.357 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.072 * | |
| Total number of observations | 172393 * | |
| Number of reflections | 29252 | |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 98.6 | 93.5 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 15 * | pH 4.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 2 | 1 | reservoir | acetate | 100 (mM) | |
| 3 | 1 | drop | protein | 15 (mg/ml) | |
| 4 | 1 | drop | Tris-HCl | 5 (mM) |
