1BZ9
CRYSTAL STRUCTURE OF MURINE CLASS I MHC H2-DB COMPLEXED WITH A SYNTHETIC PEPTIDE P1027
Replaces: 1A9DExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-07 |
Detector | RIGAKU RAXIS |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.350, 109.190, 57.780 |
Unit cell angles | 90.00, 122.81, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.266 |
Rwork | 0.252 |
R-free | 0.31900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hoc |
RMSD bond length | 0.009 |
RMSD bond angle | 25.550 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE (IN CCP4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.091 | 0.292 |
Total number of observations | 62278 * | |
Number of reflections | 11820 | |
<I/σ(I)> | 20.4 | 5.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.3 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | used microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 25 (%) | |
2 | 1 | reservoir | 150 (mM) | ||
3 | 1 | reservoir | Gly | 0.8 (M) | |
4 | 1 | reservoir | DMSO | 6 (%) | |
5 | 1 | reservoir | MES | 25 (mM) | |
6 | 1 | drop | protein | 10 (mg/ml) | |
7 | 1 | drop | Gly | 0.8 (M) | |
8 | 1 | drop | MES | 25 (mM) |