1BZ8

TRANSTHYRETIN (DEL VAL122)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	296
Detector technology	IMAGE PLATE
Collection date	1996-07-01
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 2
Unit cell lengths	43.330, 86.750, 65.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.193
Rwork	0.193
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tsh
RMSD bond length	0.011
RMSD bond angle	2.010
Data reduction software	bioteX
Data scaling software	bioteX
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.100	1.920
High resolution limit [Å]	1.800	1.800
Rmerge	0.082	0.230
Number of reflections	17380
<I/σ(I)>	6.5	1.7
Completeness [%]	74.0	56.5
Redundancy	2.7	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		pH 6.50