1BZ4

APOLIPOPROTEIN E3 (APO-E3), TRUNCATION MUTANT 165

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	125
Detector technology	AREA DETECTOR
Collection date	1997-05-15
Detector	ADSC
Spacegroup name	P 21 21 21
Unit cell lengths	40.783, 53.200, 84.777
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 1.850
R-factor	0.207
Rwork	0.207
R-free	0.24500
RMSD bond length	0.006
RMSD bond angle	1.000
Data reduction software	UCSD-system
Data scaling software	UCSD-system
Phasing software	SOLVE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.980
High resolution limit [Å]	1.840	1.840
Rmerge	0.037	0.199
Total number of observations	81518 *
Number of reflections	16493
<I/σ(I)>	27.4	3.7
Completeness [%]	99.1	93.4 *
Redundancy	4.9	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6		RT, 50MM NA-CACODYLATE, PH 5.6, 20-25% PEG 400, 1% 2-ME. NOTE : W/O 2-ME, ANOTHER ORTHORHOMBIC FORM APPEARS (SEE PDB ENTRY 1oR2).

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG400	20-25 (%)
2	1	reservoir	sodium cacodylate	50 (mM)
3	1	drop		20 (mM)
4	1	drop	protein	5 (mg/ml)