1BZ1
HEMOGLOBIN (ALPHA + MET) VARIANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-09-18 |
| Detector | SDMS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.300, 83.600, 53.800 |
| Unit cell angles | 90.00, 99.40, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.590 |
| R-factor | 0.17 * |
| Rwork | 0.170 |
| R-free | 0.22400 |
| Structure solution method | OTHER |
| Starting model (for MR) | HBA STRUCTURE PDB FILE IN BNL-25483 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.030 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.720 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.053 | 0.196 |
| Total number of observations | 399325 * | |
| Number of reflections | 61802 | |
| <I/σ(I)> | 14 | 2.48 |
| Completeness [%] | 83.9 | 66.6 |
| Redundancy | 6.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 6.5 | 2.3 M AMMONIUM SULFATE 0.3 M AMMONIUM PHOSPHATE PH 6.5 10 MM FERROUS CITRATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium phosphate | 10 (mM) |
