1BZ0
HEMOGLOBIN A (HUMAN, DEOXY, HIGH SALT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1990-06-27 |
| Detector | SDMS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.300, 83.600, 53.800 |
| Unit cell angles | 90.00, 99.40, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.500 |
| Rwork | 0.167 |
| R-free | 0.20800 |
| Structure solution method | OTHER |
| Starting model (for MR) | HBA STRUCTURE IN PDB FILE 2HHB |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.031 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.620 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.052 | 0.166 |
| Total number of observations | 828088 * | |
| Number of reflections | 84641 | |
| <I/σ(I)> | 15.6 | 3.25 |
| Completeness [%] | 95.8 | 80.1 |
| Redundancy | 9.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | Perutz, M.F., (1968) J. Cryst. Growth, 2, 54. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 4 (M) | |
| 2 | 1 | 1 | 2 (M) | ||
| 3 | 1 | 1 | 2 (M) |
