1BYU
CANINE GDP-RAN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-29 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.790, 60.433, 61.200 |
| Unit cell angles | 90.00, 101.67, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.150 |
| Rwork | 0.176 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SCHEFFZEK ET AL |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.030 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.300 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.030 | 0.097 |
| Total number of observations | 45779 * | |
| Number of reflections | 22596 | |
| <I/σ(I)> | 20.9 | 7.6 |
| Completeness [%] | 94.7 | 92.2 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 18 * | SEE PUBLICATION, pH 7.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG1000 | 20-35 (%(v/v)) | |
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | reservoir | Tris-HCl | 50 (mM) |
