1BYP
E43K,D44K DOUBLE MUTANT PLASTOCYANIN FROM SILENE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01-15 |
| Detector | RIGAKU |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 55.900, 55.900, 68.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| R-factor | 0.18 |
| Rwork | 0.188 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1PCY |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.022 |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.750 |
| Rmerge | 0.056 |
| Total number of observations | 52627 * |
| Number of reflections | 10737 |
| Completeness [%] | 93.6 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 2.8M AMMONIUM SULFATE, 100MM POTASSIUM PHOSPHATE, pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.8 (M) | |
| 2 | 1 | reservoir | potassium phosphate | 100 (mM) | |
| 3 | 1 | drop | protein | 13 (mg/ml) |
